Calpain inhibitors prevent p38 MAPK activation and germ cell apoptosis after heat stress in pubertal rat testes

Carlos Lizama, Carlos F. Lagos, Raúl Lagos-Cabré, Lorena Cantuarias, Freddy Rivera, Patricio Huenchuñir, Tomás Pérez-Acle, Flavio Carrión, Ricardo D. Moreno

Research output: Contribution to journalReview articlepeer-review

36 Citations (Scopus)

Abstract

Testicular injuries like torsion or cryptorchidism can cause massive germ cell death, which could have great impact on male reproductive health. In addition, it has been proposed that modern life style, in the form of underwear or sedentary work position, could increase the testicular temperature, induce germ cell apoptosis, reduce spermatozoa quality and promote male infertility. In this work we showed that a heat stress stimulus induced massive germ cells apoptosis, which was associated with p38 mitogen-activated protein kinase (MAPK) phosphorylation along with an increase in the levels of mRNA encoding calpain 2. Synthetic calpain inhibitors prevented heat stress-induced germ cell apoptosis through inhibition of p38 MAPK phosphorylation. Thus, our results indicate that the blockage of calpains suppresses p38 MAPK phosphorylation, and identifies calpain activation (most likely calpain 2) as an early event in heat stress-induced male germ cell apoptosis.

Original languageEnglish
Pages (from-to)296-305
Number of pages10
JournalJournal of Cellular Physiology
Volume221
Issue number2
DOIs
Publication statusPublished - Nov 2009

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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