Bovine (Bos taurus) humoral immune response against Echinococcus granulosus and hydatid cyst infertility

Rodolfo Paredes, Pablo Godoy, Betsabé Rodríguez, María Pía García, Carolina Cabezón, Gonzalo Cabrera, Verónica Jiménez, Ulf Hellman, Leonardo Sáenz, Arturo Ferreira, Norbel Galanti

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Echinococcus granulosus, the agent of hydatid disease, presents an indirect life cycle, with canines (mainly dogs) as definitive hosts, and herbivores and human as intermediary ones. In intermediary hosts fertile and infertile cysts develop, but only the first ones develop protoscoleces, the parasite form infective to definitive hosts. We report the presence of bovine IgGs in the germinal layer from infertile cysts (GLIC), in an order of magnitude greater than in the germinal layer from fertile cysts (GLFC). When extracted with salt solutions, bovine IgGs from GLIC are associated with low or with high affinity (most likely corresponding to non specific and antigen specific antibodies, respectively). Specific IgGs penetrate both the cells of the germinal layer and HeLa cultured cells and recognize parasitic proteins. These results, taken together with previous ones from our laboratory, showing induction of apoptosis in the germinal layer of infertile hydatid cysts, provide the first coherent explanation of the infertility process. They also offer the possibility of identifying the parasite antigens recognized, as possible targets for immune modulation.

Original languageEnglish
Pages (from-to)189-199
Number of pages11
JournalJournal of Cellular Biochemistry
Volume112
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • Cyst fertility
  • Cystic echinococcosis
  • Echinococcus granulosus
  • Hydatid cysts
  • Protoscolex

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Bovine (Bos taurus) humoral immune response against Echinococcus granulosus and hydatid cyst infertility'. Together they form a unique fingerprint.

Cite this