Biochemical characterization of tellurite-reducing activities of Bacillus stearothermophilus V

H. Moscoso, C. Saavedra, C. Loyola, S. Pichuantes, C. Vásquez

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Bacillus stearothermophilus V is a naturally occurring Gram-positive rod which exhibits resistance to potassium tellurite. Crude extracts of this bacterium catalyse the NADH-dependent, protease-sensitive reduction of K2TeO3 in vitro. Two fractions which showed the ability to reduce potassium tellurite (H1 and H2) were obtained. Fraction H1 behaved as a macroaggregate exhibiting a very high molecular mass that could not be estimated accurately. Upon electrophoresis in polyacrylamide gels in the presence of SDS, however, it was resolved into three distinct bands of 60, 41 and 37.5 kDa. On the other hand, an M(r) of 121 was determined for fraction H2 by means of gel filtration and high-pressure liquid chromatography. In SDS-PAGE a unique protein band of 60 kDa was observed, suggesting that it is actually a dimer. Both fractions showed pH and temperature optima of 7.5 and 57°C, respectively. Concentrations of 2.5 M NaCl or 0.35 mM SDS inhibited fraction H2 almost completely, while fraction H1 retained 20% of its activity under the same conditions. Concentrations of 5 mM EDTA caused the activity of both fractions to increase 2-fold. In addition to reducing tellurite, they were also able to reduce Na2SeO3 and Na2SO3 in vitro.

Original languageEnglish
Pages (from-to)389-397
Number of pages9
JournalResearch in Microbiology
Volume149
Issue number6
DOIs
Publication statusPublished - Jun 1998

Keywords

  • Reduction, Toxicity, Resistance, H1 and H2 fractions
  • Tellurite, Oxyanion, Thermophilic bacterium

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Molecular Biology
  • Medicine(all)

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