Behaviour of RNA and protein synthesis during the acclimatization of the Carp. Studies with isolated hepatocytes

Lino Saez, Oscar Goicoechea, Rodolfo Amthauer, Manuel Krauskopf

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

1. 1. Carp hepatocytes were isolated by dissociating the liver tissue with collagenase. The procedure yields viable cells with highly preserved ultrastructural and metabolic features. The isolated cells were able to self-aggregate and form tissue. 2. 2. RNA and protein synthesis activity was significantly higher in the carp hepatocytes from summer acclimatized fish compared to the activity present in the cold adapted animal. 3. 3. RNA synthesis assayed in carp hepatocytes suspensions obtained from summer and winter acclimatized fish exhibited a behaviour consistent with an inverse compensation to the cold acclimatization state, being apparently repressed, whereas protein synthesis did not show a compensatory activity.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume72
Issue number1
DOIs
Publication statusPublished - 1982

ASJC Scopus subject areas

  • Medicine(all)

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