Azorella compacta infusion activates human immune cells and scavenges free radicals in vitro

Lenka Tumová, Zuzana Dučaiová, José Cheel, Ivan Vokřál, Beatriz Sepúlveda, Doris Vokurková

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Background: Azorella compacta is traditionally used in the form of tea (infusion), in the Andean region of South America, to treat various chronic diseases. However, the health-promoting properties of this herbal tea have not yet been extensively explored. Materials and Methods: The free radical scavenging activity of A. compacta infusion (ACI) was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and superoxide anion radical assays. The activation of immune cells by ACI, as determined by cell surface cluster of differentiation 69 expression, was measured by flow cytometry. The qualitative polyphenolic composition of ACI was investigated by HPLC/PDA/ESI-MS, (High-performance liquid chromatography coupled with photodiode array detection and electrospray ionization-mass spectrometry) and the total content of polyphenols was estimated by spectrophotometric methods. Results: Eight polyphenols including chlorogenic acid, 6,8-di-C-hexosyl apigenin, isoorientin, orientin, dicaffeoylquinic acid, biochanin A-O-glucoside, biochanin A-O-(malonyl)-glucoside, and licoisoflavone A were tentatively identified in ACI. The total contents of phenols, flavonoids, and tannins in lyophilized ACI were 5.40 mg/100 mg ACI, 1.79 mg/100 mg ACI, and 1.76 mg/100 mg ACI, respectively. ACI, within the range of 25-400 μg/mL, scavenged DPPH and O2 .- by 15-90% and 20-88%, respectively. The human natural killer (NK) cells were substantially activated by ACI, whereas T cells and granulocytes were slightly stimulated. Conclusion: Overall, the results demonstrate the free radical scavenging and immune-stimulating properties of ACI, and support, at least in part, its potential utilization as a functional herbal tea. for preventing chronic diseases and as a nonspecific immune stimulator during human immunosenescence. Abbreviations used: ESI: electrospray ionization, HPLC: high performance liquid chromatography, PDA: photodiode array detector, MS: mass spectrometry, MS/MS: Tandem mass spectrometry, MW: molecular weight, m/z: mass-To-charge ratio, FITC: fluorescent isothiocyanate, PE: phycoerythrin.

Original languageEnglish
Pages (from-to)260-264
Number of pages5
JournalPharmacognosy Magazine
Volume13
Issue number50
DOIs
Publication statusPublished - 1 Apr 2017
Externally publishedYes

Keywords

  • Azorella compacta
  • CD69 expression
  • free radicals scavenging activity
  • immune cells
  • phenolic

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery

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