Are all nucleotidyl transferases metalloenzymes?

Pablo Valenzuela, Robert W. Morris, Anthony Faras, Warren Levinson, William J. Rutter

Research output: Contribution to journalArticle

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Abstract

Previous studies (1,2) have shown that DNA polymerases (sea urchin and E. coli I) and E. coli RNA polymerases are zinc metalloproteins. We report here that DNA-dependent RNA polymerases I and II from rat liver, I, II and III from sea urchin, and RNA-dependent DNA polymerase (reverse transcriptase) from Rous sarcoma virus are inhibited by pre-incubation with the chelating agent, o-phenanthroline. These enzymes are not affected by pre-incubation with the related compound, m-phenanthroline, which is not a metal chelating agent. The data suggest that, in addition to Mg++ or Mn++, the enzymes require a tightly bound metal ion (possibly zinc) for catalytic activity. The DNA-terminal transferase is also inhibited by o-phenanthroline but not m-phenanthroline (3). On the other hand, we found that tRNA-nucleotidyl transferases from Rous sarcoma virions and yeast are not affected by either o- or m-phenanthroline. These observations suggest that many but not all nucleotidyl transferases are metallo-enzymes.

Original languageEnglish
Pages (from-to)1036-1041
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume53
Issue number3
DOIs
Publication statusPublished - 6 Aug 1973

Fingerprint

Phenanthrolines
Transferases
Sea Urchins
RNA-Directed DNA Polymerase
Chelating Agents
Escherichia coli
Zinc
Enzymes
Avian Sarcoma
Metalloproteins
RNA Polymerase I
Rous sarcoma virus
RNA Polymerase II
DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Transfer RNA
Viruses
Virion
Liver
Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Valenzuela, P., Morris, R. W., Faras, A., Levinson, W., & Rutter, W. J. (1973). Are all nucleotidyl transferases metalloenzymes? Biochemical and Biophysical Research Communications, 53(3), 1036-1041. https://doi.org/10.1016/0006-291X(73)90196-4
Valenzuela, Pablo ; Morris, Robert W. ; Faras, Anthony ; Levinson, Warren ; Rutter, William J. / Are all nucleotidyl transferases metalloenzymes?. In: Biochemical and Biophysical Research Communications. 1973 ; Vol. 53, No. 3. pp. 1036-1041.
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Valenzuela, P, Morris, RW, Faras, A, Levinson, W & Rutter, WJ 1973, 'Are all nucleotidyl transferases metalloenzymes?', Biochemical and Biophysical Research Communications, vol. 53, no. 3, pp. 1036-1041. https://doi.org/10.1016/0006-291X(73)90196-4

Are all nucleotidyl transferases metalloenzymes? / Valenzuela, Pablo; Morris, Robert W.; Faras, Anthony; Levinson, Warren; Rutter, William J.

In: Biochemical and Biophysical Research Communications, Vol. 53, No. 3, 06.08.1973, p. 1036-1041.

Research output: Contribution to journalArticle

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