Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves

Sophie Paradis, Ana Laura Villasuso, Susana Saez Aguayo, Régis Maldiney, Yvette Habricot, Christine Zalejski, Estela Machado, Bruno Sotta, Emile Miginiac, Emmanuelle Jeannette

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.

Original languageEnglish
Pages (from-to)357-362
Number of pages6
JournalPlant Physiology and Biochemistry
Volume49
Issue number3
DOIs
Publication statusPublished - 1 Mar 2011

Keywords

  • Abscisic acid
  • Arabidopsis
  • Diacylglycerol kinase
  • Lipid phosphate phosphatase
  • Phosphatidic acid
  • Phosphatidic acid kinase
  • Stomatal movements

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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    Paradis, S., Villasuso, A. L., Aguayo, S. S., Maldiney, R., Habricot, Y., Zalejski, C., Machado, E., Sotta, B., Miginiac, E., & Jeannette, E. (2011). Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves. Plant Physiology and Biochemistry, 49(3), 357-362. https://doi.org/10.1016/j.plaphy.2011.01.010