Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth

Ana R. Sede, Cecilia Borassi, Diego L. Wengier, Martín A. Mecchia, José M. Estevez, Jorge P. Muschietti

Research output: Contribution to journalLetterpeer-review

38 Citations (Scopus)

Abstract

Proper cell wall assembly is crucial during pollen tube growth. Leucine-rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline-rich glycoprotein (HRGP) family. They contain a conserved N-terminal leucine-rich repeat (LRR) domain and a highly variable C-terminal extensin domain. Here, we characterized four LRX proteins (LRX8 through LRX11) from pollen of Arabidopsis thaliana. To investigate the role of LRX8-LRX11 in pollen germination and pollen tube growth, multiple T-DNA lrx mutants were obtained. The lrx mutants display abnormal pollen tubes with an irregular deposition of callose and pectin. They also show serious alterations in pollen germination and segregation ratio. Our results suggest that LRXs are involved in ensuring proper cell wall assembly during pollen tube growth.

Original languageEnglish
Pages (from-to)233-243
Number of pages11
JournalFEBS Letters
Volume592
Issue number2
DOIs
Publication statusPublished - Jan 2018
Externally publishedYes

Keywords

  • callose
  • cell wall
  • extensin
  • LRX
  • pectin
  • polarized growth
  • pollen tube

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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