Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature

Javier Martínez Pacheco; Philippe Ranocha; Luciana Kasulin; Corina M. Fusari; Lucas Servi; Ariel A. Aptekmann; Victoria Berdion Gabarain; Juan Manuel Peralta; Cecilia Borassi; Eliana Marzol; Diana Rosa Rodríguez-Garcia; Yossmayer del Carmen Rondón Guerrero; Mariana Carignani Sardoy; Lucía Ferrero; Javier F. Botto; Claudio Meneses; Federico Ariel; Alejandro D. Nadra; Ezequiel Petrillo; Christophe Dunand; José M. Estevez

doi:10.1038/s41467-022-28833-4

Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature

Javier Martínez Pacheco, Philippe Ranocha, Luciana Kasulin, Corina M. Fusari, Lucas Servi, Ariel A. Aptekmann, Victoria Berdion Gabarain, Juan Manuel Peralta, Cecilia Borassi, Eliana Marzol, Diana Rosa Rodríguez-Garcia, Yossmayer del Carmen Rondón Guerrero, Mariana Carignani Sardoy, Lucía Ferrero, Javier F. Botto, Claudio Meneses, Federico Ariel, Alejandro D. Nadra, Ezequiel Petrillo, Christophe DunandJosé M. Estevez

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26 Citations (Scopus)

Abstract

Root Hairs (RHs) growth is influenced by endogenous and by external environmental signals that coordinately regulate its final cell size. We have recently determined that RH growth was unexpectedly boosted when Arabidopsis thaliana seedlings are cultivated at low temperatures. It was proposed that RH growth plasticity in response to low temperature was linked to a reduced nutrient availability in the media. Here, we explore the molecular basis of this RH growth response by using a Genome Wide Association Study (GWAS) approach using Arabidopsis thaliana natural accessions. We identify the poorly characterized PEROXIDASE 62 (PRX62) and a related protein PRX69 as key proteins under moderate low temperature stress. Strikingly, a cell wall protein extensin (EXT) reporter reveals the effect of peroxidase activity on EXT cell wall association at 10 °C in the RH apical zone. Collectively, our results indicate that PRX62, and to a lesser extent PRX69, are key apoplastic PRXs that modulate ROS-homeostasis and cell wall EXT-insolubilization linked to RH elongation at low temperature.

Original language	English
Article number	1310
Journal	Nature Communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-28833-4
Publication status	Published - Dec 2022

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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Pacheco, J. M., Ranocha, P., Kasulin, L., Fusari, C. M., Servi, L., Aptekmann, A. A., Gabarain, V. B., Peralta, J. M., Borassi, C., Marzol, E., Rodríguez-Garcia, D. R., del Carmen Rondón Guerrero, Y., Sardoy, M. C., Ferrero, L., Botto, J. F., Meneses, C., Ariel, F., Nadra, A. D., Petrillo, E., ... Estevez, J. M. (2022). Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature. Nature Communications, 13(1), Article 1310. https://doi.org/10.1038/s41467-022-28833-4

@article{146ef9aaf5894c688f3fe171a66dde9d,

title = "Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature",

abstract = "Root Hairs (RHs) growth is influenced by endogenous and by external environmental signals that coordinately regulate its final cell size. We have recently determined that RH growth was unexpectedly boosted when Arabidopsis thaliana seedlings are cultivated at low temperatures. It was proposed that RH growth plasticity in response to low temperature was linked to a reduced nutrient availability in the media. Here, we explore the molecular basis of this RH growth response by using a Genome Wide Association Study (GWAS) approach using Arabidopsis thaliana natural accessions. We identify the poorly characterized PEROXIDASE 62 (PRX62) and a related protein PRX69 as key proteins under moderate low temperature stress. Strikingly, a cell wall protein extensin (EXT) reporter reveals the effect of peroxidase activity on EXT cell wall association at 10 °C in the RH apical zone. Collectively, our results indicate that PRX62, and to a lesser extent PRX69, are key apoplastic PRXs that modulate ROS-homeostasis and cell wall EXT-insolubilization linked to RH elongation at low temperature.",

author = "Pacheco, {Javier Mart{\'i}nez} and Philippe Ranocha and Luciana Kasulin and Fusari, {Corina M.} and Lucas Servi and Aptekmann, {Ariel A.} and Gabarain, {Victoria Berdion} and Peralta, {Juan Manuel} and Cecilia Borassi and Eliana Marzol and Rodr{\'i}guez-Garcia, {Diana Rosa} and {del Carmen Rond{\'o}n Guerrero}, Yossmayer and Sardoy, {Mariana Carignani} and Luc{\'i}a Ferrero and Botto, {Javier F.} and Claudio Meneses and Federico Ariel and Nadra, {Alejandro D.} and Ezequiel Petrillo and Christophe Dunand and Estevez, {Jos{\'e} M.}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1038/s41467-022-28833-4",

language = "English",

volume = "13",

journal = "Nature Communications",

issn = "2041-1723",

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Pacheco, JM, Ranocha, P, Kasulin, L, Fusari, CM, Servi, L, Aptekmann, AA, Gabarain, VB, Peralta, JM, Borassi, C, Marzol, E, Rodríguez-Garcia, DR, del Carmen Rondón Guerrero, Y, Sardoy, MC, Ferrero, L, Botto, JF, Meneses, C, Ariel, F, Nadra, AD, Petrillo, E, Dunand, C & Estevez, JM 2022, 'Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature', Nature Communications, vol. 13, no. 1, 1310. https://doi.org/10.1038/s41467-022-28833-4

TY - JOUR

T1 - Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature

AU - Pacheco, Javier Martínez

AU - Ranocha, Philippe

AU - Kasulin, Luciana

AU - Fusari, Corina M.

AU - Servi, Lucas

AU - Aptekmann, Ariel A.

AU - Gabarain, Victoria Berdion

AU - Peralta, Juan Manuel

AU - Borassi, Cecilia

AU - Marzol, Eliana

AU - Rodríguez-Garcia, Diana Rosa

AU - del Carmen Rondón Guerrero, Yossmayer

AU - Sardoy, Mariana Carignani

AU - Ferrero, Lucía

AU - Botto, Javier F.

AU - Meneses, Claudio

AU - Ariel, Federico

AU - Nadra, Alejandro D.

AU - Petrillo, Ezequiel

AU - Dunand, Christophe

AU - Estevez, José M.

PY - 2022/12

Y1 - 2022/12

N2 - Root Hairs (RHs) growth is influenced by endogenous and by external environmental signals that coordinately regulate its final cell size. We have recently determined that RH growth was unexpectedly boosted when Arabidopsis thaliana seedlings are cultivated at low temperatures. It was proposed that RH growth plasticity in response to low temperature was linked to a reduced nutrient availability in the media. Here, we explore the molecular basis of this RH growth response by using a Genome Wide Association Study (GWAS) approach using Arabidopsis thaliana natural accessions. We identify the poorly characterized PEROXIDASE 62 (PRX62) and a related protein PRX69 as key proteins under moderate low temperature stress. Strikingly, a cell wall protein extensin (EXT) reporter reveals the effect of peroxidase activity on EXT cell wall association at 10 °C in the RH apical zone. Collectively, our results indicate that PRX62, and to a lesser extent PRX69, are key apoplastic PRXs that modulate ROS-homeostasis and cell wall EXT-insolubilization linked to RH elongation at low temperature.

AB - Root Hairs (RHs) growth is influenced by endogenous and by external environmental signals that coordinately regulate its final cell size. We have recently determined that RH growth was unexpectedly boosted when Arabidopsis thaliana seedlings are cultivated at low temperatures. It was proposed that RH growth plasticity in response to low temperature was linked to a reduced nutrient availability in the media. Here, we explore the molecular basis of this RH growth response by using a Genome Wide Association Study (GWAS) approach using Arabidopsis thaliana natural accessions. We identify the poorly characterized PEROXIDASE 62 (PRX62) and a related protein PRX69 as key proteins under moderate low temperature stress. Strikingly, a cell wall protein extensin (EXT) reporter reveals the effect of peroxidase activity on EXT cell wall association at 10 °C in the RH apical zone. Collectively, our results indicate that PRX62, and to a lesser extent PRX69, are key apoplastic PRXs that modulate ROS-homeostasis and cell wall EXT-insolubilization linked to RH elongation at low temperature.

UR - http://www.scopus.com/inward/record.url?scp=85126672733&partnerID=8YFLogxK

U2 - 10.1038/s41467-022-28833-4

DO - 10.1038/s41467-022-28833-4

M3 - Article

C2 - 35288564

AN - SCOPUS:85126672733

SN - 2041-1723

VL - 13

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 1310

ER -

Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature

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