Antioxidant effects of an ozonized theobroma oil formulation on damaged-inflammatory rat skin

Yaima Sánchez, Maritza F. Díaz, Frank Hernández, Dayana Gil, Gastón García

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The aim of this study was to determine whether a cosmetic formulation elaborated with ozonized theobroma oil may exert beneficial effects in the restoring of the antioxidant activity on the skin of rats previously irradiated with ultraviolet light. 0.5 g of the formulation was applied on the skin of rats for five days. Superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activity were determined in a homogenate of rat skin. Malondialdehyde (MDA), conjugated dienes (CD) and total hydroperoxide (THP) content were determined as biomarkers of oxidative stress. Using these parameters, antioxidant and oxidant activity, redox index and oxidative stress grade were determined. The total antioxidant activity was significantly increased while the redox index, total oxidant activity and oxidative stress grade decreased significantly in damaged rats treated with the formulation. These results show the antioxidant properties of the cosmetic formulation due to the stimulation of antioxidant enzymes such as SOD and GPx, preventing skin injury induced by ultraviolet irradiation.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalGrasas y Aceites
Issue number1
Publication statusPublished - Jan 2011
Externally publishedYes


  • Antioxidant activity
  • Cosmetic formulation
  • Oxidant activity
  • Oxidative stress
  • Ozonized theobroma oil

ASJC Scopus subject areas

  • Food Science
  • Organic Chemistry


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