An increased expression of nucleolin is associated with a physiological nucleolar segregation

M. Alvarez, C. Quezada, C. Navarro, A. Molina, P. Bouvet, M. Krauskopf, M. I. Vera

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Nucleolar segregation is the most striking cellular phenotypic feature of cold-acclimatized carp and depicts the cyclical reprogramming that the physiology of the fish undergoes between summer and winter, where a clear differential expression of some nucleolar related genes occurs. We characterized carp nucleolin, a nucleolar protein involved in multiple steps of ribosome biogenesis, and evaluated its expression upon fish acclimatization. We show that the carp cDNA deduced amino acid sequence exhibits the same tripartite structural organization found in other species. Nevertheless, we observed that nucleolin mRNA expression was strongly induced in the cold-adapted carp as was the nuclear protein content, assessed by immunocytochemistry in liver sections. The physiological up-regulation of nucleolin in the cold-acclimatized carp, where rRNA transcription and processing are depressed concomitantly with the nucleolus segregation, is consistent with the notion that nucleolin plays a fundamental role in repressing rRNA synthesis.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 31 Jan 2003


  • Acclimatization
  • Adaptation
  • Carp
  • Fish
  • Nucleolin
  • Nucleolus
  • Ribosomal protein L41
  • U3 snoRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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