TY - JOUR
T1 - Adoptive transfer of autoimmune splenic dendritic cells to lupus-prone mice triggers a B lymphocyte humoral response
AU - Sauma, Daniela
AU - Crisóstomo, Natalia
AU - Fuentes, Camila
AU - Gleisner, María Alejandra
AU - Hidalgo, Yessia
AU - Fuenzalida, María José
AU - Rosemblatt, Mario
AU - Bono, María Rosa
N1 - Funding Information:
Animal work was carried out under the institutional regulations of the Fundaci?n Ciencia & Vida and was approved locally by the ethical review committee of the Facultad de Ciencias, Universidad de Chile. Funded by FONDECYT 1140431 (MRB), FONDECYT 1121478 (DS), PAI 791100009 (MRB, DS), FONDEQUIP/EQM114137, and CONICYT PFB-16 (MR). The authors declare that they have no conflict of interest.
Publisher Copyright:
© 2017, The Author(s).
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by increased autoantibody production that leads to multiple tissue injuries. Dendritic cells (DCs) are important orchestrators of immune responses and key components in fine-tuning the balance between tolerance and immunity. However, their role in autoimmune disorders such as SLE remains uncertain. We analyzed the contribution of DCs in triggering SLE by adoptively transferring splenic DCs from aged autoimmune [NZB×NZW]F1 (BWF1) mice to young healthy BWF1 mice. We observed that the transfer of DCs from autoimmune mice to pre-autoimmune mice induced high autoantibody titers in the serum of recipient mice. Moreover, autoimmune DCs from aged BWF1 mice were crucial for the expansion and differentiation of plasmablasts and CD5+ B cells or B1-like cells in the peripheral blood, and spleen of recipient BWF1 mice, a phenomenon that is observed in autoimmune BWF1 mice. On the other hand, DCs from aged BWF1 mice participated in the expansion and differentiation of DCs and IFN-γ-producing T cells. These results reveal that DCs from autoimmune BWF1 mice exhibit functional and phenotypic characteristics that allow them to trigger B cell hyperactivation, as well as DC and T cell expansion and differentiation, thereby promoting an exacerbated humoral response in lupus-prone mice.
AB - Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by increased autoantibody production that leads to multiple tissue injuries. Dendritic cells (DCs) are important orchestrators of immune responses and key components in fine-tuning the balance between tolerance and immunity. However, their role in autoimmune disorders such as SLE remains uncertain. We analyzed the contribution of DCs in triggering SLE by adoptively transferring splenic DCs from aged autoimmune [NZB×NZW]F1 (BWF1) mice to young healthy BWF1 mice. We observed that the transfer of DCs from autoimmune mice to pre-autoimmune mice induced high autoantibody titers in the serum of recipient mice. Moreover, autoimmune DCs from aged BWF1 mice were crucial for the expansion and differentiation of plasmablasts and CD5+ B cells or B1-like cells in the peripheral blood, and spleen of recipient BWF1 mice, a phenomenon that is observed in autoimmune BWF1 mice. On the other hand, DCs from aged BWF1 mice participated in the expansion and differentiation of DCs and IFN-γ-producing T cells. These results reveal that DCs from autoimmune BWF1 mice exhibit functional and phenotypic characteristics that allow them to trigger B cell hyperactivation, as well as DC and T cell expansion and differentiation, thereby promoting an exacerbated humoral response in lupus-prone mice.
KW - Autoimmunity
KW - B lymphocytes
KW - Dendritic cells
KW - Humoral response
KW - Systemic lupus erythematosus
UR - http://www.scopus.com/inward/record.url?scp=85025638003&partnerID=8YFLogxK
U2 - 10.1007/s12026-017-8936-9
DO - 10.1007/s12026-017-8936-9
M3 - Article
AN - SCOPUS:85025638003
VL - 65
SP - 957
EP - 968
JO - Immunologic Research
JF - Immunologic Research
SN - 0257-277X
IS - 4
ER -