Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritimum, a member of the Cytophaga-Flavobacterium-Bacteroides (CFB) group

Manuel Romero, Rubén Avendaño-Herrera, Beatriz Magariños, Miguel Cámara, Ana Otero

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

Tenacibaculum maritimum (formerly Flexibacter maritimus) is a filamentous, biofilm-forming member of the Cytophaga-Flavobacterium-Bacteroides group (or Bacteroidetes), which causes the widely distributed marine fish disease tenacibaculosis. A search for N-acylhomoserine lactones (AHLs) quorum-sensing (QS) signals in the culture media of nine representative strains of this species using different biosensor strains revealed the presence of short-type AHL activity in all of them. N-butyryl-l-homoserine lactone (C4-HSL) was identified in T. maritimum NCIMB2154T by LC-MS. A degradation activity for long-acyl AHLs (C10-HSL) was subsequently demonstrated in T. maritimum NCIMB2154T. The acidification of the culture medium after degradation did not allow the recovery of C10-HSL, which indicates a possible acylase-type degradation activity. Even though the physiological processes under the control of AHL-mediated QS in T. maritimum need to be further characterized, this discovery extends the paradigm of AHL-mediated QS signalling beyond the Proteobacteria and reinforces its ecological significance.

Original languageEnglish
Pages (from-to)131-139
Number of pages9
JournalFEMS Microbiology Letters
Volume304
Issue number2
DOIs
Publication statusPublished - Mar 2010

Keywords

  • Acylhomoserine lactone
  • Cytophaga- Flavobacterium-Bacteroides
  • Quorum quenching
  • Quorum sensing

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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