Activation of the template activity of isolated rat liver nuclei for DNA synthesis and its inhibition by NAD

L. Burzio, S. S. Koide

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

The template activity of isolated rat liver nuclei for DNA synthesis assayed with E.coli DNA polymerase was found to be dependent upon the presence of Ca2+ or Mg2+ in the incubation medium. DNA was prepared from isolated nuclei subjected to conditions which activated the template and centrifuged in an alkaline sucrose gradient. The distribution profile showed that smaller fragments were formed, suggesting enhancement of endonucleolytic activity. When isolated nuclei were incubated with NAD to induce poly(adenosine diphosphate ribose) formation and were subjected to the activation conditions, the template for DNA synthesis remained unchanged. The distribution profile in an alkaline sucrose gradient of DNA prepared from these nuclei and control nuclei was identical. The present findings suggest that the template-activating system for DNA synthesis was blocked when isolated nuclei were treated with NAD invitro.

Original languageEnglish
Pages (from-to)572-579
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume53
Issue number2
DOIs
Publication statusPublished - 17 Jul 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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