A simple one-step method for the preparation of highly purified formylmethionine transfer ribonucleic acid and methionine transfer ribonucleic acid from Escherichia coli

C. Henes, M. Krauskopf, J. Ofengand

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Phenoxyacetylation of methionyl transfer ribonucleic acid of Escherichia coli and fractionation on BD-cellulose at 4° was shown to be a rapid and convenient method for obtaining highly purified formylmethionine transfer ribonucleic acid and methionine ribonucleic acid well separated from each other. In a single run 80% pure formylmethionine transfer ribonucleic acid and 35 % pure methionine ribonucleic acid were obtained. If the chromatography was performed at 22°, ribonucleic acids specific for methionine could also be obtained highly purified but the isoaccepting species were not separated from each other. Rechromatography of material from the 4° column resulted in further purification of both ribonucleic acids, approaching 100% for formylmethionine transfer ribonucleic acid and about 70% for methionine ribonucleic acid. The order of elution of the phenoxyacetylmethionyl derivatives of formylmethionine transfer ribonucleic acid and methionine ribonucleic acid was inverted with respect to the two uncharged or aminoacylated ribonucleic acid species. It is suggested that N acylation of the methionine residue has induced a differential conformational change in the two ribonucleic acid structures which is detected by altered affinity for the BD-cellulose.

Original languageEnglish
Pages (from-to)3024-3028
Number of pages5
JournalBiochemistry
Volume8
Issue number7
Publication statusPublished - 1969

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'A simple one-step method for the preparation of highly purified formylmethionine transfer ribonucleic acid and methionine transfer ribonucleic acid from Escherichia coli'. Together they form a unique fingerprint.

  • Cite this