@inbook{76f1d15aa4964fa0835b9de68a6e08de,
title = "A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System",
abstract = "The Nobel Prize awarded gene editing system, CRISPR-Cas9, is probably one of the greatest achievements of the last decades. CRISPR-Cas9 can introduce irreversible genomic changes in its target DNA by simple specifying a 20-nucleotide sequence within its RNA guide. Due to its simplicity, efficacy, and relative low cost in comparison with other genome editing systems, it has become the most common gene editing system used in research laboratories. Here we describe a step-by-step protocol to produce genetically edited primary oral keratinocytes using the CRISPR-Cas9 system.",
keywords = "CRISPRCas9, Gene editing, Genetic engineering, Oral biology, Oral keratinocytes, Primary cell cultures",
author = "Niklander, {Sven E.} and Hunter, {Keith D.}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2780-8_14",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "217--229",
booktitle = "Methods in Molecular Biology",
}