A Method for Isolation of Intact, Translationally Active Ribonucleic Acid

Guy Cathala, Jean Francois Savouret, Bernardita Mendez, Brian L. West, Michael Karin, Joseph A. Martial, John D. Baxter

Research output: Contribution to journalArticlepeer-review

1203 Citations (Scopus)

Abstract

A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (<300 nucleotides) RNA species are recovered with a poor yield.

Original languageEnglish
Pages (from-to)329-335
Number of pages7
JournalDNA
Volume2
Issue number4
DOIs
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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