Abstract
We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174-bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage ΦP27. STY1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid-copy-vector produced retardation in S. Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl-β-d-thio-galactoside in pCC1™ single-copy vector. Our results support the fact that S. Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope.
Original language | English |
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Pages (from-to) | 58-66 |
Number of pages | 9 |
Journal | FEMS Microbiology Letters |
Volume | 321 |
Issue number | 1 |
DOIs | |
Publication status | Published - Aug 2011 |
Keywords
- Holin
- Inner membrane
- Salmonella
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics