A flow cytometric procedure for the quantification of cell adhesion in complex mixtures of cells

María Rosa Bono, Lilian I. Reyes, Mario Rosemblatt

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We present a simple non-radioactive cytometry-based assay that permits the simultaneous quantitation of cell adhesion of distinct subsets of cells contained in a mixture without any previous fractionation. The procedure is simple and highly reproducible and has the advantage of confining the quantitation of cell adhesion to live cells only. This new approach is based on counting the absolute number of cells. This is done by adding known numbers of distinguishable beads to the cell suspension and counting beads and cells in a cytometer. Quantitation of adhesion is accomplished by counting each subpopulation of cells before and after the adhesive process. To illustrate this methodology we determined adhesion of Ramos cells to monolayers of endothelial cells and its inhibition by specific antibodies. Also, we determined adhesion to endothelial cells of B lymphocytes and subsets of T lymphocytes present in a preparation of unfractionated human mononuclear cells. The results presented here demonstrate that the new assay has the required properties to be used in the quantitation of cell adhesion.

Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalJournal of Immunological Methods
Volume223
Issue number1
DOIs
Publication statusPublished - 1 Feb 1999

Keywords

  • Cell adhesion
  • Flow cytometric assay
  • Lymphocyte subpopulation adhesion analysis
  • Mixed cell adhesion analysis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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