α-Factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

A. J. Brake, J. P. Merryweather, D. G. Coit, U. A. Heberlein, F. R. Masiarz, G. T. Mullenbach, M. S. Urdea, P. Valenzuela, P. J. Barr

Research output: Contribution to journalArticlepeer-review

390 Citations (Scopus)

Abstract

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone α-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

Original languageEnglish
Pages (from-to)4642-4646
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number15 I
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • General

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